Identification of inherited protein variants in individual erythrocytes
Document Type
Article
Publication Date
6-1-1980
Abstract
Inherited electrophoretic variants of hemoglobin, carbonic anhydrase, and glucose-6-phosphate dehydrogenase in individual erythrocytes were separated by electrophoresis in ultrathin agar gels. By staining the electropherograms with specific fluorescein-conjugated antibodies against hemoglobins, relative proportions of two hemoglobins within individual erythrocytes can be estimated. The findings suggested that the intracellular proportions of HbA and HbS in heterozygotes are heterogeneous within a given population of cells. By this method cells containing hemoglobin F (F cells) as well as a minor variant of hemoglobin F were identified. This tool potentially offers an approach to monitoring distribution of inherited variants in individual erythrocytes for a large number of proteins. © 1980 Plenum Publishing Corporation.
Recommended Citation
Anyaibe, Stephen I.O. and Headings, Verle E., "Identification of inherited protein variants in individual erythrocytes" (1980). The Center For Sickle Cell Disease Faculty Publications. 327.
https://dh.howard.edu/sicklecell_fac/327
