A synthetic peptide from fibronectin inhibits experimental metastasis of murine melanoma cells
Document Type
Article
Publication Date
1-1-1986
Abstract
Adhesive interactions between cells and the extracellular matrix occur at several stages of metastasis. Such interactions might be inhibited by synthetic peptide probes derived from the cell-binding regions of matrix molecules. Gly-Arg-Gly-Asp-Ser (GRGDS) is a pentapeptide sequence that appears to be critical for cell interaction with fibronectin. Coinjection of GRGDS with B16-F10 murine melanoma cells dramatically inhibited the formation of lung colonies in C57BL/6 mice. Two closely related control peptides, in which specific amino acids within the GRGDS sequence were transposed or substituted, displayed little or no activity. Inhibition by GRGDS was dose-dependent, noncytotoxic, and did not result from an impairment of cellular tumorigenicity. GRGDS may function by inhibiting tumor cell retention in the lung since radiolabeled B16-F10 tumor cells injected with the peptide were lost at a substantially greater rate than control cells.
Recommended Citation
Humphries, Martin J.; Olden, Kenneth; and Yamada, Kenneth M., "A synthetic peptide from fibronectin inhibits experimental metastasis of murine melanoma cells" (1986). Howard University Cancer Center Faculty Publications. 285.
https://dh.howard.edu/hucancer_fac/285
