"DHPLC elution patterns of vdr PCR products can predict prostate cancer" by Robert L. Copeland, Desta Beyene et al.
 

DHPLC elution patterns of vdr PCR products can predict prostate cancer susceptibility in african american men

Document Type

Article

Publication Date

11-1-2017

Abstract

Background/Aim: Denaturing high-performance liquid chromatography (DHPLC) is a technique that is used to detect mutations. The aim of the present study was to determine whether DHPLC elution patterns of vitamin D receptor (VDR) gene PCR products can serve as indicators of susceptibility to prostate cancer (PCa) risk. Materials and Methods: DNA samples of PCa cases and controls were screened for mutations and/or polymorphisms in coding exons of VDR gene using DHPLC analysis. Logistic regression, phi-coefficient (φ), and Backward Wald models were used to analyze the data. Results: Similar elution patterns of exons 1, 6, 7 and 9 along with higher prevalence of heteroduplex DNA were observed in PCa samples than in controls. Exons 4 and 8 had highly significant protective effects (p<0.05). Whereas, exons 5, 7, and 9 were perfectly positively correlated with PCa risk (φ=1), thus presenting candidate exons significantly associated with susceptibility to PCa. Conclusion: DHPLC elution patterns of the selected exons could be useful to predict susceptibility to develop PCa.

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