Quantitation of hemoglobins by immunodiffusion: Specific antibodies to hemoglobins A1, S and F

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A simple and sensitive immunochemical method for quantifying hemoglobins A1, S and F is described. Antibodies to human hemoglobins A1, S and F were obtained in goats and after cross absorption were shown to be specific for thei corresponding hemoglobins. These specific antisera were employed in a radial immunodiffusion method to quantify hemoglobins A1, S and F in hemoglobin specimens of known phenotype as ascertained by electrophoresis on cellulose acetate. The method accurately quantifies hemoglobins in purified hemoglobin mixtures designed to simulate expected proportions in newborn infants who have sickle cell trait or sickle cell anemia. Principal application of this methodology currently under consideration are in diagnosis of hemoglobin SS in the infanct and studies on rates of development change in hemoglobins A1, S and F. © 1974.

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