Characteristics of modulators and substrates binding to rat liver adenylate kinase

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The binding of adenine nucleotides to liver adenylate kinase was dependent on Mg2+ ions. Citric acid enhanced the binding of all metal chelated radioactive nucleotides and indicated 2 observable binding sites for Mg3H ADP and Mg3H ATP and one half binding site for Mg3H AMP. Two binding sites of Mg3H ADP and one binding site for Mg3H ATP were also observed in the absence of citric acid. Stoichiometric binding of 14C citric acid to liver adenylate kinase varied with additions of different nucleotides. AMP prevented whereas ADP and ATP enhanced the binding of 14C citric acid.

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