Differences in the Biosynthesis and Localization of the Fibronectin Receptor in Normal and Transformed Cultured Human Cells
We examined the biosynthesis and localization of the fibronectin receptor integrin from normal and transformed cultured human cells. Normal cells required a minimum of 20 h for the biosynthesis of completely mature fibronectin-receptor β-subunit, while transformed cells required only 6-8 h. There was a correspondingly major decrease in the amount of the intracellular β-chain precursor in the transformants. Immunostaining of normal fibroblastic cells with monoclonal antibodies indicated that both α- and β-polypeptides of the fibronectin receptor are localized in cell surface streaks and focal contact areas. In contrast, both subunits lacked this clustering and had a more diffuse distribution on the surfaces of transformed cells, even though quantitative immunofluorescence experiments indicated that similar or larger amounts of each subunit were present on a per cell basis. Both immunostaining and biochemical analyses also indicated the presence of a relatively large intracellular pool of β-polypeptides in normal fibroblasts that is not present in transformed cells. There was no major transformation-dependent change in total quantities of mature fibronectin receptor subunit expressed and inserted into the plasma membrane, when normalized to total protein synthesis. Our results indicate that malignant transformation of cultured human cells results in altered localization and processing of the fibronectin receptor. Such changes involving pathways of crucial cell surface molecules may contribute to alterations in their interactions with extracellular macromolecules, including during the process of cellular invasion. © 1990, American Association for Cancer Research. All rights reserved.
Akiyama, Steven K.; Larjava, Hannu; and Yamada, Kenneth M., "Differences in the Biosynthesis and Localization of the Fibronectin Receptor in Normal and Transformed Cultured Human Cells" (1990). Howard University Cancer Center Faculty Publications. 226.